I like using One shot for fairly static subjects such as nudibrachs or CFWA images. One shot lets me focus on the Nudi's head then recompose so I don't have the area of focus solely in the middle of the image. Same thing for CFWA shots where I want to put the closest object in the image lower on the frame. Howver, if I have time, I will switch to the other focus points on the camera instead of doing the focus then recompose method.
AI Servo works great with moving fish, pygmy seahorses etc. If the focus engine of the Canon DSLRs are rated to follow a fast moving car, then a fish is well within the capabilities, though when you're trying to get a good shot of an anemone fish, you'd swear that wasn't the case!

It would help if you let the camera find focus first on the subject for a second or two before firing. For pygmy seahorses, I find that AI servo is great because the tracking system compensates for the my slight bouyancy changes as I breathe in and out.
Stu